Figure 8.

(A) SNG-treatment inhibited thyrosphere formation of PTC cells. BCPAP cells were grown and treated in ultralow attachment plates with 0 µM or 0.5 µM, 1 µM and 2 µM of SNG for 7 days, and images of thyrospheres were taken on day 7 using an EVOS FLc cell imaging system from Invitrogen (Thermo Fisher Scientific) at a magnification of 4× (scale bar 1000 µm). (B) BCPAP and (C) TPC-1 cells spheres were grown and treated in ultralow attachment plates with 0 µM, 1 µM, 2 µM and 4 µM of SNG for 3 days, followed by cell lysis and Western blotting with antibodies against ALDH2, SOX-2 and HSP60.