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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: Curr Biol. 2019 Sep 26;29(19):3256–3265.e5. doi: 10.1016/j.cub.2019.08.032

Figure 3. Interactions of LLG2/3 with Ecto-domains of BUPS1/2 and ANX1/2.

Figure 3.

(A) Dual-membrane yeast two-hybrid assays showing interaction of LLG2/3 with ectodomains of BUPS1/2 and ANX1/2. Yeast cells containing indicated plasmids were plated on the growth medium (-Leu-Trp) or on selection medium (-Leu-Trp-His-Ade).

(B and C) Interactions between LLG2/3 with BUPS1/2 (B) and ANX1/2 (C) ectodomains by pull-down assays. LLG2/3 were expressed in E. coli, and ectodomains of BUPS1/2 and ANX1/2 were transiently generated in tobacco leaves. Pull-down assays were performed with Ni Sepharose, and western blots were probed with antibodies against FLAG and His (α-FLAG or α-His), respectively.

Similar results were obtained by three independent experiments.