KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| ProteinFind Anti-His Mouse Monoclonal Antibody | TransGen Biotech | HT501-02 |
| anti-Flag-HRP | Sigma-Aldrich | A8592-2MG |
| Goat Anti-Mouse IgG-HRP | CWBIO | CW0102S |
| Anti-MBP Monoclonal Antibody | NEB | E8038S |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Biotin-RALF4 | Scilight Biotechnology | N/A |
| RALF4 | Scilight Biotechnology | N/A |
| Biotin-RALF4-N19 | Scilight Biotechnology | N/A |
| RALF4-N19 | Scilight Biotechnology | N/A |
| Biotin-RALF4-N29 | Scilight Biotechnology | N/A |
| RALF4-N29 | Scilight Biotechnology | N/A |
| Biotin-RALF4-N48 | Scilight Biotechnology | N/A |
| RALF4-N48 | Scilight Biotechnology | N/A |
| Biotin-RALF4m | Scilight Biotechnology | N/A |
| RALF4m | Scilight Biotechnology | N/A |
| Biotin-RALF4-C34 | Scilight Biotechnology | N/A |
| RALF4-C34 | Scilight Biotechnology | N/A |
| Biotin-RALF4-C24 | Scilight Biotechnology | N/A |
| RALF4-C24 | Scilight Biotechnology | N/A |
| Biotin-RALF4-C11 | Scilight Biotechnology | N/A |
| RALF4-C11 | Scilight Biotechnology | N/A |
| Biotin-RALF4-C5 | Scilight Biotechnology | N/A |
| RALF4-C5 | Scilight Biotechnology | N/A |
| Biotin-RALF19 | Scilight Biotechnology | N/A |
| RALF19 | Scilight Biotechnology | N/A |
| Biotin-RALF23 | Scilight Biotechnology | N/A |
| RALF23 | Scilight Biotechnology | N/A |
| Biotin-RALF34 | Scilight Biotechnology | N/A |
| RALF34 | Scilight Biotechnology | N/A |
| 3xFlag Peptide | Sigma | F4799-4 MG |
| SuperSignal@West Femto Maximum Sensitivity Substrate | Thermo Fisher | 34095 |
| Copmplete Protease Inhib. Cocktail | Roche | 11836145001 |
| Ni Sepharose 6 FF | GE Healthcare | 17531801 |
| Anti-Flag Affinity Gel | Bimake | B23101 |
| Streptavidin Magnetic Particles | Spherotech | SVMS-30-10 |
| Amylose Resin | NEB | E8021L |
| Experimental Models: Organisms/Strains | ||
| Arabidopsis thaliana: Col0 | ABRC | N/A |
| Arabidopsis thaliana: llg2-1 | This study | N/A |
| Arabidopsis thaliana: llg2-2 | This study | N/A |
| Arabidopsis thaliana: llg2-3 | This study | N/A |
| Arabidopsis thaliana: llg3-1 | This study | N/A |
| Arabidopsis thaliana: llg3-2 | This study | N/A |
| Arabidopsis thaliana: llg3-3 | This study | N/A |
| Arabidopsis thaliana: bups1-2 bups2-1 | [14] | N/A |
| Arabidopsis thaliana: LLG2p::GUS | This study | N/A |
| Arabidopsis thaliana: LLG3p::GUS | This study | N/A |
| Arabidopsis thaliana: ms1 | [31] | N/A |
| Arabidopsis thaliana: LLG2p::SP-GFP-LLG2 (llg2 llg3) | This study | N/A |
| Arabidopsis thaliana: Lat52:SP-YFP-LLG3 | This study | N/A |
| Nicotiana tabacum cultivar SR 1 | This study | N/A |
| Oligonucleotides | ||
| Primers used in this study, see Table S1 | This study | N/A |
| Recombinant DNA | ||
| Plasmid LLG2p::GUS | This study | N/A |
| Plasmid LLG3p::GUS | This study | N/A |
| Plasmid pHEE401E-LLG2-LLG3 | This study | N/A |
| Plasmid pENTR-LLG2-LLG3 | This study | N/A |
| Plasmid pET28GW-LLG1 | This study | N/A |
| Plasmid pET28GW-LLG2 | This study | N/A |
| Plasmid pET28GW-LLG3 | This study | N/A |
| Plasmid pMALGW-ectoFER | This study | N/A |
| Plasmid 35Sp::ectoBUPS1-Flag | [14] | N/A |
| Plasmid 35Sp::ectoBUPS2-Flag | [14] | N/A |
| Plasmid 35Sp::ectoANX1-Flag | [14] | N/A |
| Plasmid 35Sp::ectoANX2-Flag | [14] | N/A |
| Plasmid LLG2p::SP-GFP-LLG2 | This study | N/A |
| Plasmid Lat52:SP-YFP-LLG3 | This study | N/A |
| Plasmid Lat52:SP-mCitrine-LLG2 | This study | N/A |
| Plasmid Lat52:SP-mCitrine-LLG3 | This study | N/A |
| Software and Algorithms | ||
| Arabidopsis Information Resource | http://www.arabidopsis.org/ | N/A |
| Morpheus | https://software.broadinstitute.org/morpheus | N/A |
| UniProt | https://www.uniprot.org/ | N/A |
| MEGA 6.0 | http://www.megasoftware.net/ | N/A |
| ImageJ | https://imagej.nih.gov/ij/ | N/A |