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. 2008 Sep 10;51(4):217–223. doi: 10.1159/000154258

Fig. 2.

Fig. 2

Expression of IFN-α subtype mRNAs in PBMCs from 10 healthy donors after SARS coronavirus (CoV, HSR1 strain) infection at a multiplicity of infection of 0.1 (a). The same experiments were conducted with VSV and NDV viruses (b, c). At both 24 and 48 h after infection, cells were harvested and RNA was isolated using TRIzol reagent. TaqMan reverse transcriptase PCR was used to quantify IFN-α subtype mRNAs. The data, normalized to β-glucuronidase mRNA, were calculated by using the arithmetic formula 2−δδCt according to the supplier's guidelines. * IFN q = IFN-α5, −10, −17, and −21. a ** p < 0.05 compared with expression of IFN-α subtypes in uninfected PBMCs (controls) using Student's t test. *** p < 0.05 compared with expression of IFN-α subtypes in uninfected PBMCs (controls) and at 24 h p.i. using Student's t test. b, c ** p < 0.05 compared with expression of IFN-α subtypes in PBMCs infected with SARS-CoV using Student's t test.