Fig. 2.

2′-5′-Oligoadenylate synthetase and p56 are up-regulated in Vero cells following treatment with type I IFN and IFN-γ. Vero cells were exposed to either IFN-α, IFN-β, IFN-γ or both type I IFNs (IFN-α/IFN-β) and IFN-γ for 18–20 h. Cells were harvested and RNA isolated 18–20 h after exposure. TaqMan reverse transcriptase polymerase chain reaction was used to quantify 2′-5′-oligoadenylate synthetase (OAS) and p56 mRNA expression. Data, normalised to hypoxanthine phosphoribosyltransferase mRNA, were calculated by using the arithmetic formula ₂ – ΔΔCt according to the supplier's guidelines. * p < 0.05 compared with fold change of 2′-5′-oligoadenylate synthetase and p56 after exposure of Vero cells to IFN-α, IFN-β or IFN-γ separately using Student's t test.