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. 2011 Mar 3;55(1):53–61. doi: 10.1159/000323523

Table 1.

Sequences of template deoxynucleotides for siRNAs used for target genes

Gene targets Sequences Position
T7 promoter 5′-TAATACGACTCACTATAGGAGACAGG-3′

SRI
Sense
Antisense
5′-GCAAATACTCTGACTATAA-3′
5′-AATTATAGTCAGAGTATTTGCCCTGTCTC-3′
5′-AAGCAAATACTCTGACTATAACCTGTCTC-3′
12361-12379

SR2
Sense
Antisense
5′-GCACAAGTTCTTAGTGAAA-3′
5′-AATTTCACTAAGAACTTGTGCCCTGTCTC-3′
5′-AAGCACAAGTTCTTAGTGAAACCTGTCTC-3′
15282-15300

SS1
Sense
Antisense
5′-TACACTATGTGTGAATACC-3′
5′-AAGGTATTCACACATAGTGTACCTGTCTC-3′
5′-AATACACTATGTGTGAATACCCCTGTCTC-3′
23910-23928

SS2
Sense
Antisense
5′-TTTCTGTGGTAATGGTAAT-3′
5′-AAATTACCATTACCACAGAAACCTGTCTC-3′
5′-AATTTCTGTGGTAATGGTAATCCTGTCTC-3′
26789-26807

Scramble siRNA
Sense
Antisense
5′-ACATAAACCTATCAGTAGT-3′
5′-AAACTACTGATAGGTTTATGTCCTGTCTC-3′
5′-AAACATAAACCTATCAGTAGTCCTGTCTC-3′

The 8 nucleotide underlined sequences of template deoxynucleotides for each siRNA are complemented with those of the T7 promoter primer.