Table 1.
Gene targets | Sequences | Position |
---|---|---|
T7 promoter | 5′-TAATACGACTCACTATAGGAGACAGG-3′ | |
SRI Sense Antisense |
5′-GCAAATACTCTGACTATAA-3′ 5′-AATTATAGTCAGAGTATTTGCCCTGTCTC-3′ 5′-AAGCAAATACTCTGACTATAACCTGTCTC-3′ |
12361-12379 |
SR2 Sense Antisense |
5′-GCACAAGTTCTTAGTGAAA-3′ 5′-AATTTCACTAAGAACTTGTGCCCTGTCTC-3′ 5′-AAGCACAAGTTCTTAGTGAAACCTGTCTC-3′ |
15282-15300 |
SS1 Sense Antisense |
5′-TACACTATGTGTGAATACC-3′ 5′-AAGGTATTCACACATAGTGTACCTGTCTC-3′ 5′-AATACACTATGTGTGAATACCCCTGTCTC-3′ |
23910-23928 |
SS2 Sense Antisense |
5′-TTTCTGTGGTAATGGTAAT-3′ 5′-AAATTACCATTACCACAGAAACCTGTCTC-3′ 5′-AATTTCTGTGGTAATGGTAATCCTGTCTC-3′ |
26789-26807 |
Scramble siRNA Sense Antisense |
5′-ACATAAACCTATCAGTAGT-3′ 5′-AAACTACTGATAGGTTTATGTCCTGTCTC-3′ 5′-AAACATAAACCTATCAGTAGTCCTGTCTC-3′ |
The 8 nucleotide underlined sequences of template deoxynucleotides for each siRNA are complemented with those of the T7 promoter primer.