Fig. 3.

NF-κB activation by viral fusion peptides. Effect of peptides on binding of NF-κB subunits to an NF-κB-binding consensus sequence. U937 cells were stimulated with viral peptides at 10 μM. Cell lysates were tested for binding of the activated p50 or p65 subunits to an NF-κB consensus sequence using the Trans-Am NF-κB ELISA kit. The experiment was performed in the presence of soluble wild-type or mutated consensus oligonucleotides. The results are expressed as specific binding (absorbance measured in the presence of the mutated oligonucleotide minus that measured in the presence of the wild-type oligonucleotide). The results are shown as means 8 SEs of triplicate determinations.