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. 2008 Nov 21;51(5):342–351. doi: 10.1159/000175837

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Copyright © 2008 by S. Karger AG, Basel

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Fig. 1. a — Amino acid sequence alignment of TCoV nsp15 with selected nsp15 sequences from other coronaviruses. * Denotes key residues associated with NendoU activity. b, c Expression of TCoV nsp15: b SDS-PAGE analysis of nsp15 purification from BL21 (DE3) cells. M = Molecular weight marker (Invitrogen); Sup = cleared cell lysate; FL = flow-through after passing through Ni-NTA column; W1 and W2, washes; E, elutes; c Western blot of purified nsp15 with mouse anti-His mAb and detected with goat anti-mouse IgG-HRP. Arrows indicate the nsp15.