Fig. 5. a.

Effect of Mn²⁺ on activity of nsp15. nsp15 was incubated with different concentrations of Mn²⁺ for 10 min on ice and then RNA was added and reaction was incubated for 60 min at 37°. b Effect of divalent metal ions on nsp15 activity. nsp15 was incubated with 5 mM of different ions for 10 min on ice and then RNA was added and reaction was incubated for 60 min at 37°. c Effect of preincubation of nsp15 with EDTA on nsp15 activity. Purified TCoV nsp15 was incubated with 0, 1, 5, and 50 mM EDTA for 10 min on ice and then RNA substrate was added and the reaction was incubated for 30 min at 37°.