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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: J Am Chem Soc. 2019 Sep 26;141(40):15747–15750. doi: 10.1021/jacs.9b08036

Figure 1.

Figure 1.

(A) Optical design and (B) schematic of the confocal-feedback 3D-SMT system. By comparing the differences in photon counts measured by 4 detection channels, the 3D position of the tracked molecule is estimated. A feedback algorithm then drives the piezo stage to bring the molecule back to the center of confocal volumes. Thus, the motion history of the xyz piezo stage represents the 3D trajectory of the tracked molecule. (C) Composite lifetime traces are different with and without the quencher strand.