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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: J Am Chem Soc. 2019 Sep 26;141(40):15747–15750. doi: 10.1021/jacs.9b08036

Figure 2.

Figure 2.

Using a time-correlated single-photon counting (TCSPC) module, we can time tag the arrival photons and group them into consecutive time windows for lifetime analysis. Here “1” represents the annealing/bound state while “2” represents the melting/unbound state. A hidden Markov model is then used to identify the state transitions in the lifetime trace.