FIG 1.
Schematic representation of insertion of the ISEcp1-blaCTX-M-14 tandem into the K. pneumoniae S54 chromosome leading to the formation of a hybrid promoter. (A) Original sequence found in a wild-type K. pneumoniae genome. (B) Sequence identified in isolate 54 once the ISEcp1-blaCTX-M-14 tandem has been acquired by transposition. Arrows show promoters that are described in detail at the bottom. The lysR gene encodes a putative LysR regulator; the fosA gene encodes the FosA glutathione S-transferase; the mdaB gene encodes an NAD(P)H-dependent oxidoreductase. Promoter −35 and −10 sequences are boxed. IRL, inverted long repeat; DR, direct repeat; RBS, ribosome binding site; +1, start codon of the lysR gene.