FIG 5.
Characterization of the six tet(X)- and blaNDM-1-coharboring plasmids by the PacBio RSII platform. (a) Species, source, and chromosome size of each strain are indicated. The transposition unit TU1 of tet(X) and the transposition unit Tn125 (TU2) of blaNDM-1 are indicated. (b) Linear sequence comparison of the six tet(X)- and blaNDM-1-coharboring plasmids. The results of sequence alignment were generated with Easyfig, version 2.1. Regions of homology are marked by shading. The delta (Δ) symbol indicates a truncated gene. Genes associated with plasmid replication, antimicrobial resistance, heavy metal resistance, and mobility are marked in green, red, orange, and blue, respectively, while other gene are shown in gray. (c) A radial representation of a phylogenetic tree of six tet(X)- and blaNDM-1-coharboring plasmids and homologs. (d) Hypothesis of the molecular evolution of the six tet(X)- and blaNDM-1-coharboring plasmids.