TABLE 1.
Comparison of the in vitro susceptibility of Plasmodium knowlesi (clone A1-H.1) and Plasmodium falciparum (clone 3D7) exposed to novel endochin-like quinolones for one complete life cycle
| Compound | EC50 (nM)a |
Fold differenceb | Pc | |
|---|---|---|---|---|
| P. knowlesi A1-H.1 (27-h exposure) | P. falciparum 3D7 (48-h exposure) | |||
| Endochin | 18.9 ± 1.2 | 18.1 ± 0.5 | 1.04 | 0.6233 |
| ELQ-271 | 117 ± 12 | 64.5 ± 3.1 | 1.81 | 0.0081 |
| ELQ-300 | 15.4 ± 0.9 | 23.1 ± 1.2 | 0.67 | 0.0215 |
| ELQ-316 | 47.1 ± 2.6 | 33.5 ± 2.3 | 1.41 | 0.0097 |
| ELQ-331 | 49.0 ± 6.2 | 45.4 ± 1.6 | 1.08 | 0.5014 |
| ELQ-400 | 6.80 ± 0.26 | 4.95 ± 0.26 | 1.37 | 0.0030 |
| ELQ-480 | 7.06 ± 0.32 | 5.81 ± 0.26 | 1.22 | 0.0433 |
| Chloroquine | 33.1 ± 2.0 | 17.7 ± 1.3 | 1.87 | <0.0001 |
| Dihydroartemisinin | 1.52 ± 0.07 | 3.64 ± 0.42 | 0.42 | 0.0112 |
a
Means ± standard errors of the means from at least 4 experiments, each performed in duplicate.
b
Calculated as P. knowlesi EC50/P. falciparum EC50.
c
Calculated by comparing EC50 values for P. knowlesi versus P. falciparum using Student’s two-tailed paired t test.