FIG 2.
C. parapsilosis MGXr 5-2 has a defect in mitochondrial function. (A) C. parapsilosis MGXr 5-2 forms petite colonies on YPD agar and does not grow on YP-glycerol agar; 10-fold dilutions of stationary-phase cultures of C. parapsilosis were spotted on YP agar containing 2% (wt/vol) d-glucose or glycerol and then photographed after 48 h of growth at 30°C. (B) C. parapsilosis MGXr 5-2 has reduced mitochondrial membrane potential when subcultured in medium containing 2% (wt/vol) glycerol. MitoTracker Red CMXRos fluorescence was monitored by flow cytometry. Data are median fluorescence intensities (DsRed) ± SDs from 3 independent experiments (10,000 events/sample). Differences between groups were determined by ratio paired t test. *, P ≤ 0.05. (C) Representative micrographs of MitoTracker Red-stained cells prepared the same as for those in panel B.