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. 2020 Apr 21;64(5):e02284-19. doi: 10.1128/AAC.02284-19

FIG 5.

FIG 5

Growth dynamics and morphological changes of Candida and Cryptococcus grown in the presence of LF, AMB, and LF-AMB. Time-kill curves of C. albicans SC5314 (A) and C. neoformans H99 (B) incubated at 35°C with shaking at 180 rpm and either left untreated or treated with the LF-D1 FIC, AMB FIC, or LF-AMB FIC averaged across three independent replicates. OD, optical density. (C) Scanning electron microscopy images of the four yeast species after 48 h (C. albicans SC5314, C. glabrata CBS138) or 72 h (C. neoformans H99, C. deuterogattii R265) of growth untreated or in the presence of 1/2 MIC of LF-D1 or AMB or 1/2 FIC of LF-AMB (the MICs and FICs for each species can be found in Table 2). Magnifications = ×2,000 for C. albicans, C. neoformans, and C. deuterogattii and ×4,000 for C. glabrata. Bars = 5 μm for C. albicans, C. neoformans, and C. deuterogattii and 2.5 μm for C. glabrata. White arrowheads indicate the morphological changes following treatment.