TABLE 3.
Effect of iron saturation on antifungal activity of LF and synergy with AMB
| Lactoferrin source | Treatment (name) | Iron saturation (%)a |
C. albicans SC5314 |
C. glabrata CBS138 |
C. neoformans H99 |
C. deuterogattii R265 |
||||
|---|---|---|---|---|---|---|---|---|---|---|
| MIC (μg/ml) | FICIb | MIC (μg/ml) | FICIb | MIC (μg/ml) | FICIb | MIC (μg/ml) | FICIb | |||
| Sigma | Control (LF-S) | 27.7 | >256 | 0.5 | >256 | 0.5 | >256 | 0.5 | >256 | 0.5 |
| Saturated (holo-LF-S) | 78.3 | >256 | 0.5 | >256 | 0.5 | >256 | 0.5 | >256 | 0.5 | |
| Dialyzed (apo-LF-S) | 5.2 | 256 | 0.5 | 256 | 0.5 | 256 | 0.5 | 128 | 0.5 | |
| Dairy 1 | Control (LF-D1) | 14.5 | 16 | 0.375 | 16 | 0.5 | 16 | 0.5 | 8 | 0.375 |
| Saturated (holo-LF-D1) | 72.9 | >256 | 0.375 | >256 | 0.5 | >256 | 0.5 | >256 | 0.375 | |
| Dialyzed (apo-LF-D1) | 1.1 | 32 | 0.375 | 32 | 0.5 | 32 | 0.5 | 16 | 0.375 | |
| Dairy 2 | Control (LF-D2) | 10.3 | 16 | 0.375 | 16 | 0.5 | 16 | 0.5 | 8 | 0.375 |
| Saturated (holo-LF-D2) | 71.4 | >256 | 0.375 | >256 | 0.5 | >256 | 0.5 | >256 | 0.375 | |
| Dialyzed (apo-LF-D2) | 2.6 | 32 | 0.375 | 32 | 0.5 | 32 | 0.5 | 16 | 0.375 | |
a
Iron saturation was determined by ICP-MS.
b
FICI of LF from the specified source plus AMB. Values in bold indicate synergistic activity.