FIG 1.

Optimal seeding densities of B. mandrillaris trophozoites for drug susceptibility testing in vitro in 96- and 384-well plates. RLU was determined in white plates, microscopy was performed in clear plates. Using RLU production, timing of the logarithmic phase, and cell health as determined by microscopy, for 96-well plates (left) we determined an optimum of 16,000 B. mandrillaris per well and for 384-well plates (right) we determined an optimum of 4,000 B. mandrillaris per well for 72 h experimentation within all future drug experiments.