Figure 2.

AlCl₃·decreases cell viability and increases apoptosis in PC12 cells. (A) The neurotoxic effect of AlCl₃ with different concentrations (1, 2, 4, 5, 6, 8, and 10 mM) was determined by an MTT assay. (B) The effects of AlCl₃ incubation at 4 mM for different hours (6, 12, 24, and 48 h) on cell viability were assessed by an MTT assay. (C) After 48 h incubation, AlCl₃-induced apoptosis was determined with annexin-V/PI staining, followed by FACS analysis. The bar graph on the right shows the percentages of cells in early and late apoptosis. Values are means ± standard derivation from three independent experiments. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. 0 group.