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. 2020 Apr 9;8:180. doi: 10.3389/fcell.2020.00180

FIGURE 1.

FIGURE 1

OPA1-Exon4b is not required for mitochondrial fusion but ensues mitochondrial bioenergetic recovery. (A) Labeling of one subset of mitochondria by photoactivation of PAGFP in cells expressing both mtPAGFP and mtDsRed, as indicated on the top (scale bar: 10 μm). (B) Quantitation of mitochondrial fusion events, including kiss-and-run and complete fusion, per run (n = 10 runs). (C) Δψm of WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. The mean TMRM FI of mitochondria in WT cells was normalized to 1. The TMRM FI per mitochondrion below 0.3 denoted low (white arrow). (D) Quantitation of percentage of mitochondria with low Δψm in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag (≥20 cells for three biological replicates). (E) Quantitation of percentage of mitochondria with low Δψm in Opa1 KO cells expressing ShLuc plus Flag and Opa1 KO cells expressing OPA1-iso5-Flag plus ShLuc, ShMfn1, ShMfn2 or ShMfn1/2 (≥20 cells for three biological replicates). **p < 0.01, one-way ANOVA.