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. 2020 Apr 9;8:180. doi: 10.3389/fcell.2020.00180

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Copyright © 2020 Yang, Tang, Lin, Wu, Zeng, Pan, Li, Xiang, Lin, Zhuang, Song, Jiang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OPA1 Exon-4b and Exon4 partially recover mitochondrial respiration activity. (A) OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag. (B) Relative ATP production, basal, and maximal respiration using OCR measurements in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag or OPA1-iso5-Flag (n = 2, biological replicates). WT MEF cells were used as control, whose ATP production, basal, and maximal respiration were normalized to 1. (C) Cellular ATP levels in WT MEF cells expressing Flag and Opa1 KO cells expressing Flag, OPA1-iso1-Flag, or OPA1-iso5-Flag, with or without oligomycin treatment (n = 3, biological replicates). *p < 0.05, **p < 0.01, one-way ANOVA.