TABLE 2.
Sequences of primer pairs used in this study.
| Primer | Sequence (5′–3′) |
| TmPGRP-LE_qPCR_Fw | 5′-CTTCGCTTGCGGAATGGCAGATTA-3′ |
| TmPGRP-LE_qPCR_Rv | 5′-AACACACGCTCAAATCCTTTCCCG-3′ |
| dsTmPGRP-LE_Fw | 5′-TAATACGACTCACTATAGGGAGGCAACGT |
| AAATAAGGACGG-3′ | |
| dsTmPGRP-LE_Rv | 5′-TAATACGACTCACTATAGGGAGTAGGCGA |
| TATCGTTCCACTTC-3′ | |
| dsEGFP_Fw | 5′-TAATACGACTCACTATAGGGTACGTAAAC |
| GGCCACAAGTTC-3′ | |
| dsEGFP_Rv | 5′-TAATACGACTCACTATAGGGTTGCTCAGG |
| TAGTGTTGTCG-3′ | |
| TmTenecin-1_Fw | 5′-CAGCTGAAGAAATCGAACAAGG-3′ |
| TmTenecin-1_Rv | 5′-CAGACCCTCTTTCCGTTACAGT-3′ |
| TmTenecin-2_Fw | 5′-CAGCAAAACGGAGGATGGTC-3′ |
| TmTenecin-2_Rv | 5′-CGTTGAAATCGTGATCTTGTCC-3′ |
| TmTenecin-3_Fw | 5′-GATTTGCTTGATTCTGGTGGTC-3′ |
| TmTenecin-3_Rv | 5′-CTGATGGCCTCCTAAATGTCC-3′ |
| TmTenecin-4_Fw | 5′-GGACATTGAAGATCCAGGAAAG-3′ |
| TmTenecin-4_Rv | 5′-CGGTGTTCCTTATGTAGAGCTG-3′ |
| TmDefensin-1_Fw | 5′-AAATCGAACAAGGCCAACAC-3′ |
| TmDefencin-1_Rv | 5′-GCAAATGCAGACCCTCTTTC-3′ |
| TmDefensin-2_Fw | 5′-GGGATGCCTCATGAAGATGTAG-3′ |
| TmDefensin-2_Rv | 5′-CCAATGCAAACACATTCGTC-3′ |
| TmColeoptericin-1_Fw | 5′-GGACAGAATGGTGGATGGTC-3′ |
| TmColeoptericin-1_Rv | 5′-CTCCAACATTCCAGGTAGGC-3′ |
| TmColeoptericin-2_Fw | 5′-GGACGGTTCTGATCTTCTTGAT-3′ |
| TmColeoptericin-2_Rv | 5′-CAGCTGTTTGTTTGTTCTCGTC-3′ |
| TmAttacin-1a_Fw | 5′-GAAACGAAATGGAAGGTGGA-3′ |
| TmAttacin-1a_Rv | 5′-TGCTTCGGCAGACAATACAG-3′ |
| TmAttacin-1b_Fw | 5′-GAGCTGTGAATGCAGGACAA-3′ |
| TmAttacin-1b_Rv | 5′-CCCTCTGATGAAACCTCCAA-3′ |
| TmAttacin-2_Fw | 5′-AACTGGGATATTCGCACGTC-3′ |
| TmAttacin-2_Rv | 5′-CCCTCCGAAATGTCTGTTGT-3′ |
| TmCecropin-2_Fw | 5′-TACTAGCAGCGCCAAAACCT-3′ |
| TmCecropin-2_Rv | 5′-CTGGAACATTAGGCGGAGAA-3′ |
| TmThaumatin-like protein-1_Fw | 5′-CTCAAAGGACACGCAGGACT-3′ |
| TmThaumatin-like protein-1_Rv | 5′-ACTTTGAGCTTCTCGGGACA-3′ |
| TmThaumatin-like protein-2_Fw | 5′-CCGTCTGGCTAGGAGTTCTG-3′ |
| TmThaumatin-like protein-2_Rv | 5′-ACTCCTCCAGCTCCGTTACA-3′ |
| TmRelish_qPCR_Fw | 5′-AGCGTCAAGTTGGAGCAGAT-3′ |
| TmRelish_qPCR_Rv | 5′-GTCCGGACCTCATCAAGTGT-3′ |
| TmDorX1_qPCR_Fw | 5′-AGCGTTGAGGTTTCGGTATG-3′ |
| TmDorX1_qPCR_Rv | 5′-TCTTTGGTGACGCAAGACAC-3′ |
| TmDorX2_qPCR_Fw | 5′-ACACCCCCGAAATCACAAAC-3′ |
| TmDorX2_qPCR_Rv | 5′-TTTCAGAGCGCCAGGTTTTG-3′ |
| TmL27a_qPCR_Fw | 5′-TCATCCTGAAGGCAAAGCTCCAGT-3′ |
| TmL27a_qPCR_Rv | 5′-AGGTTGGTTAGGCAGGCACCTTTA-3′ |
Underline indicates T7 promotor sequences.