FIGURE 1.

Castanospermine significantly inhibits Zika virus (ZIKV) replication in Vero and CHME3 cells without cytotoxicity. Vero cells (A) and CHME3 cells (B) were treated with castanospermine in concentrations ranging from 0.01 to 1000 μM, and cytotoxicity was quantified by MTS assay at 72 h post treatment. Absorbance was measured at 490 nm. Cell culture supernatant from Vero cells (C,E) and CHME3 cells (D,F) infected with ZIKV PRVABC59 at MOI of 0.5 or 5 with and without 1 μM castanospermine treatment were harvested at 48 hpi. ZIKV RNA levels were determined by qRT-PCR assay (C,D). Infectious ZIKV titration was determined by TCID₅₀ assay (E,F). Data represent mean values of four biological replicates. Error bars represent standard deviation.