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. 2020 Apr 7;11:531. doi: 10.3389/fmicb.2020.00531

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Copyright © 2020 Bhushan, Lim, Bird, Chothe, Nissly and Kuchipudi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Deoxynojirimycin (DNJ) significantly inhibits Zika virus (ZIKV) replication in Vero and CHME3 cells without cytotoxicity. Vero cells (A) and CHME3 cells (B) were treated with DNJ in concentrations ranging from 0.01 to 1000 μM, and cytotoxicity was quantified by MTS assay at 72 h post treatment. Absorbance was measured at 490 nm. Cell culture supernatant from Vero cells (C,E) and CHME3 cells (D,F) infected with ZIKV PRVABC59 at MOI of 0.5 or 5 with and without 1 μM DNJ treatment were harvested at 48 hpi. ZIKV RNA levels were determined by qRT-PCR assay (C,D). Infectious ZIKV titration was determined by TCID₅₀ assay (E,F). Data represent mean values of four biological replicates. Infectious ZIKV titration was determined by TCID₅₀ assay (E,F). Error bars represent standard deviation.