Skip to main content
. 2020 Apr 16;11:606. doi: 10.3389/fimmu.2020.00606

FIGURE 2.

FIGURE 2

IRF3 and IRF8 regulate MyD88-mediated NF-κB signaling pathway. (A) Epithelioma papulosum cyprini (EPC) cells were seeded in 24-well plates and cotransfected with 0.2 μg of MyD88 and 0.2 μg of pcDNA3.1, IRF3 or IRF8, respectively, together with 0.25 μg of NF-κB, IL-1β reporter gene or IL-8 reporter gene, respectively. After 24 h post transfection, the luciferase activity was measured. (B) The concentration gradient experiment of IRF3 (0.05, 0.1, or 0.2 μg) or IRF8 (0.05, 0.1, or 0.2 μg) expression plasmid within 0.2 μg of MyD88 and 0.25 μg of NF-κB reporter gene was conducted. After 24 h post transfection, the luciferase activity was measured. After being cotransfected with MyD88 and IRF3 or IRF8 expression plasmids, together with NF-κB reporter gene, the luciferase activity was measured at different time points. The “EV” represents pcDNA3.1 empty plasmid. The luciferase activity value was achieved against the Renilla luciferase activity. *p < 0.05 versus the controls. All experiments were performed in at least three independent experiments.