a, Percentage of CD44+ CD3+ T cells, CD44+ CD8+ T cells, and CD44+ CD4+ T cells in the blood of Snrnp40swp/rplc and Snrnp40swp/+ littermates (n=3 mice per genotype; ***P=0.0009, ***P=0.0008, n.s., not significant (P=0.2833), unpaired, two-tailed Student’s t-test). b, Mean fluorescence intensity (MFI) of CD44 staining on T cells in the blood of Snrnp40swp/rplc and Snrnp40swp/+ littermates (n=3 mice per genotype; **P=0.0011, ***P=0.0004, **P=0.0096, unpaired, two-tailed Student’s t-test). c-f, Flow cytometry of lymphocytes from spleen (c-d) and lymph node (e-f) of Snrnp40swp/rplc and Snrnp40swp/+ littermates (n=3 mice per genotype), assessing the expression of CD44 and CD62L on CD8+ and CD4+ T cells (c,e). Quantification of the percentage of central memory (CD44hiCD62L+), effector memory (CD44hiCD62L–) and naïve (CD44loCD62L+) T cells (d,f). ***P=0.00046, **P=0.0012, ***P=0.00049, **P=0.0016, **P=0.0013; n.s., not significant (P=0.055) in d, and ***P=0.00012, ***P=0.00081, ****P=0.000066, **P=0.006, ****P=0.00002, ****P=0.000061 in f (unpaired, two-tailed Student’s t-test). g, Flow cytometry of blood or spleen cells from Snrnp40swp/rplc and Snrnp40swp/+ mice, assessing the expression of CD3ε on T cells. Quantification of MFI of CD3ε staining on T cells is shown (inset) (n=3 mice per genotype; ***P=0.0001, ***P=0.0010, unpaired, two-tailed Student’s t-test). h,i, Flow cytometry assessing the proliferation (h) and apoptosis (i) of adoptively transferred CD8+ and CD4+ T cells in the spleens of recipient mice. An equal mixture of CellTrace™ Far Red-labeled pan T cells isolated from the spleens of Snrnp40swp/rplc and Snrnp40swp/+ mice was adoptively transferred into irradiated C57BL/6J wild-type hosts. Histograms showing CellTrace™ Far Red dilution in cells harvested from spleens of irradiated or non-irradiated hosts seven days after transfer (h). Percentage of annexin V-positive T cells four days after transfer (i) (n=5 recipient mice; **P=0.0046, **P=0.0015, unpaired, two-tailed Student’s t-test). Data are representative of two (h,i), three (c-f), five (a,b), or six (g) independent experiments (mean ± s.d. in a,b,d,f,i).