Table 4.

sgRNA and ss DN sequences used to introduce the S37F and K335I mutations into cell lines. The lowercase nucleotides are silent mutations in the ss DNs that will alter the gRNA recognition sequence to prevent re-cutting. nderlined and bold A will introduce the S37F and K335I mutation.

sgRNA S37F	gCT GGACT CTGGAATCCATT C
sgRNA K335I	GATACTTACGAAAAACTACTG
ss DN S37F	CCACTCATACAGGACTTGGGAGGTATCCACATCCTCTTCCTCAGGATTGCCTTTACCACTCAGAGAAGGAGCTGTGGTAGTGGCACCAAAgTGaATTCCAGAGTCCAGGTAAGACTGTTGCTGCCAG
ss DN K335I	ATAGATACATATACTTACCAGCTTCTACAATAGCCGGCTTATTACTAGAGCAGACAGATAGCACCTTCAGCAGCTTGTGGTCCAtAGaAGTATTTCGTAAGTATAGGTCCTCATTATATTTAC