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. 2020 Apr 23;16(4):e1008693. doi: 10.1371/journal.pgen.1008693

Fig 4. GCN1 and GCN2 dependency in response to UV exposure.

Fig 4

(A)(B) The WT and Gcn1ΔRWDBD (A) or Gcn2 KO (Gcn2-/-) MEFs (B) were irradiated by 80 J/m2 UV and were allowed to recover in fresh medium for 30 min or 4 h before harvest. Whole cell protein extracts of the MEFs were subjected to immunoblot analysis to detect phosphorylated GCN2 (P-GCN2), GCN2, phosphorylated eIF2α (P-eIF2α), eIF2α and β-actin.