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. 2020 Apr 23;16(4):e1008464. doi: 10.1371/journal.ppat.1008464

Fig 5. Reduced CD22-/- B cell numbers in the lungs of recipient mice detected 48 hours after cellular transfer.

Fig 5

Flow cytometric analysis of lung and spleen cells from C57BL/6 mice injected i.v. with CFSE labelled C57BL/6 WT splenocytes and CTV labelled CD22-/- splenocytes (or vice versa) in a 1:1 mix. At various time points lungs and spleens were collected and stained for B220 and CD3. A) Gating strategy for the analysis of the proportion of WT and CD22-/- B and T cells found in the lung of recipient WT mice. B) Analysis of the percentage of WT and CD22-/- B and T cells found in the lung and spleen 2, 24 and 48 hours after i.v. injection. The proportion of WT and CD22-/- donor cells is displayed as the ratio of the percentage of CTV or CFSE-positive B or T cells found in the lung or spleen of recipient mice, divided by the percentage of CTV or CFSE-positive B or T cells injected (input) x 100. Bar diagrams show a summary of 11 independent experiments with 8 recipient mice per time point. Statistics were done with a two-tailed t test. ** = p<0.01.