Fig 7. CD22-deficient mice have a lower number of GM-CSF producing IRA B cells in the lungs after pneumococcal infections.

C57BL/6 control and CD22-/- mice (C57BL/6 background) were infected with 1 x 10⁵ CFU S. pneumoniae intranasally and were analysed 72 hours after infection. (A) IRA B cells (MHCII+, CD19+, CD43+, CD93+) were identified in lung and spleen, as indicated for lung. (B) IRA B cells and GM-CSF+ and intracellular IgM+ (IgMic+) B cells were quantified in the lung. (C) GM-CSF+ and intracellular IgM+ (IgMic+) B cells were quantified and in the spleen. For B) and C) total cell numbers were calculated as percentage of total leukocyte numbers. * = p<0.05. n = 7 (C57BL/6) or n = 8 (CD22-/-) mice were used. A representative of two experiments is shown.