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. 2020 Apr 13;16(4):e1008732. doi: 10.1371/journal.pgen.1008732

Fig 6. UTR extensions in endogenous genes of the pho1-7 tex1-4 and pho1-7 hpr1-6 mutants.

Fig 6

3′ (A) Illumina RNAseq reads density maps (blue) showing examples of 3’ UTR extensions for genes AT1G03160 (left) and AT3G11310 (right) in the pho1-7 tex1-4 and pho1-7 hpr1-6 mutants. The 3′ UTR extensions are depicted by a red rectangle. The exons of the genes are indicated as red boxes below. (B) Venn diagram showing the number and overlap in genes with 3’UTR extensions. (C) Validation of 3’ UTR extensions via qPCR. (D) Transient expression of Luciferase gene fused after the stop codon to the 3’end of genes AT1G76560 and AT1G03160. The constructs were expressed in Arabidopsis mesophyll protoplasts obtained from Col-0, hpr1-6 and tex1-4. The bar chart shows the relative ratios of long-to-short transcripts in the various genotypes. For C, data are means of three samples with each sample being a pool of seven seedlings and three technical replicates for each sample. For D, data are means of four samples with each sample being an independent transfection of protoplasts. Error bars represent standard deviation. Asterisks denote statistical significance (P < 0.05) from the Col-0 control according to Student’s t test (n.s. = not statistically different).