Figure 3. Comparative immunorecognition ability of the Brazilian SAB antivenom towards B. brazili and B. jararaca venom toxins. (A) Third-generation antivenomic analyses of B. brazili and (B) B. jararaca venom with the pentabothropic antivenom (soro antibotrópico, SAB) produced at Butantan Institute. Reverse-phase chromatographic analysis of whole venom (panels a) and of the non-retained and the immunoretained fractions recovered from affinity column [9 mg immobilized SAB antivenom F(ab’)₂ molecules] incubated with increasing amounts (300-3600 µg) of venom from (A) B. brazili (Pará, Brazil) and (B) B. jararaca (SE population) are displayed in panels b through i. Panels j-l show reverse-phase HPLC separations of the retained and non-retained venom fractions on mock matrix and naïve equine IgG affinity columns, respectively.