Table 1. Concentration-dependent immunoretained (RET) Bothrops brazili (Bbr) venom proteins by SAB antivenom affinity column. Maximal binding for each RP-HPLC fraction is highlighted in bold face.

Bothrops brazili total venom proteins ( µg)
RP-HPLC fraction		100	300	600	900	1200	2400	3600	Extrapolation	Toxin class
2-3	µg TOTAL	10.09	30.27	60.55	90.82	121.09	242.18	363.28		SVMPi
	µg RET	0.00	0.00	0.00	0.00	0.00	0.00	0.00
6-8	µg TOTAL	1.52	4.56	9.13	13.69	18.25	36.50	54.76	76.00	DC fragment
	µg RET	1.52	3.18	6.34	8.87	12.03	19.56	21.69	25.15
9-10	µg TOTAL	0.31	0.93	1.87	2.80	3.73	7.46	11.20		PLA2, NGF
	µg RET	0.31	0.93	1.87	2.80	3.47	2.98	2.98
11	µg TOTAL	7.93	23.80	47.60	71.40	95.20	190.39	285.59	396.50	PLA2
	µg RET	7.93	19.22	21.09	16.71	20.18	21.43	22.80	25.85
12	µg TOTAL	6.29	18.87	37.73	56.60	75.47	150.94	226.40		PLA2
	µg RET	6.29	17.01	21.99	21.90	21.06	23.05	22.45
13	µg TOTAL	1.97	5.90	11.80	17.69	23.59	47.18	70.78		PLA2
	µg RET	1.97	5.05	6.15	6.11	6.05	6.40	6.38
14	µg TOTAL	0.83	2.50	5.00	7.51	10.01	20.02	30.02		PLA2
	µg RET	0.83	2.30	3.37	3.84	4.35	3.90	3.78
15	µg TOTAL	1.61	4.82	9.63	14.45	19.26	38.52	57.78		PLA2
	µg RET	1.61	4.46	6.35	6.69	6.62	6.16	6.03
16-17	µg TOTAL	0.21	0.62	1.24	1.85	2.47	4.94	7.42	10.50	SVSP
	µg RET	0.21	0.62	1.24	1.85	2.47	2.94	3.06	4.16
18	µg TOTAL	0.26	0.77	1.54	2.31	3.08	6.17	9.25		SVSP
	µg RET	0.26	0.77	1.54	2.31	3.08	2.68	2.34
19	µg TOTAL	1.69	5.08	10.16	15.24	20.32	40.63	60.95	84.50	CRISP
	µg RET	1.69	5.08	10.16	15.24	20.32	23.73	25.24	33.56
20-22	µg TOTAL	2.61	7.82	15.64	23.45	31.27	62.54	93.82	130.50	SVSP
	µg RET	2.61	7.25	11.81	15.04	17.78	20.35	22.73	28.85
24-25	µg TOTAL	3.70	11.11	22.22	33.33	44.44	88.87	133.31		PLA2, SVSP
	µg RET	3.70	8.48	19.97	25.14	25.08	20.47	18.21
26-28	µg TOTAL	15.63	46.88	93.77	140.65	187.54	375.07	562.61		SVSP, PLA2
	µg RET	15.63	45.42	45.52	42.40	41.17	43.65	41.43
30-31	µg TOTAL	0.28	0.83	1.67	2.50	3.34	6.67	10.01		SVSP
	µg RET	0.28	0.83	1.67	2.50	3.34	3.32	3.31
33-34	µg TOTAL	1.83	5.48	10.96	16.44	21.92	43.85	65.77	91.50	SVSP
	µg RET	1.83	5.48	10.96	16.44	21.92	22.97	33.65	36.75
35	µg TOTAL	2.21	6.62	13.24	19.85	26.47	52.94	79.42	110.50	LAO
	µg RET	2.21	6.62	13.24	19.85	26.47	31.65	34.00	43.24
38	µg TOTAL	14.60	43.79	87.59	131.38	175.18	350.35	525.53	730.00	PI-SVMP
	µg RET	14.17	43.24	86.01	130.62	164.29	173.27	188.76	255.13
42-43	µg TOTAL	11.75	35.24	70.49	105.73	140.98	281.95	422.93	587.50	PI-SVMP
	µg RET	10.81	33.45	66.70	95.65	118.52	177.47	202.71	235.74
46	µg TOTAL	14.70	44.10	88.19	132.29	176.39	352.78	529.16	735.00	PIII-SVMP
	µg RET	13.64	42.81	85.65	128.28	163.15	310.34	318.67	384.76