Table 2. Concentration-dependent immunoretained (RET) Bothrops jararaca (SE) (Bj) venom proteins by SAB antivenom affinity column. Maximal binding for each RP-HPLC fraction is highlighted in bold face.

Bothrops jararaca total venom proteins ( µg)
RP-HPLC fraction		100	300	600	900	1200	2400	3600	Extrapolation	Toxin class
1	µg TOTAL	8.62	25.86	51.71	77.57	103.43	206.86	310.28	431.00	BPP + DISI
1	µg RET	0.45	2.50	5.77	9.18	9.17	9.94	10.96	15.45	BPP + DISI
2	µg TOTAL	5.03	15.09	30.19	45.28	60.37	120.74	181.12	250.15	DISI
2	µg RET	0.81	6.00	7.59	9.70	11.06	15.31	19.50	21.15	DISI
3	µg TOTAL	2.01	6.04	12.07	18.11	24.14	48.29	72.43		DISI + BPP
3	µg RET	0.14	0.20	0.29	0.38	0.48	0.51	0.51		DISI + BPP
4	µg TOTAL	0.70	2.11	4.22	6.34	8.45	16.90	25.34	35.00	DC fragment
4	µg RET	0.70	2.11	4.22	6.34	7.23	7.45	8.31	11.21	DC fragment
5	µg TOTAL	2.09	6.26	12.53	18.79	25.06	50.11	75.17	104.50	VEGF, PLA₂
5	µg RET	2.09	6.26	9.39	9.43	9.57	10.79	16.12	16.30	VEGF, PLA₂
6	µg TOTAL	1.48	4.44	8.87	13.31	17.75	35.50	53.24	74.00	CRISP
6	µg RET	1.48	4.44	8.87	12.53	16.78	30.59	34.08	40.00	CRISP
7	µg TOTAL	12.01	36.02	72.04	108.05	144.07	288.14	432.22	600.51	SVSP, PLA₂, CTL
7	µg RET	12.01	36.02	72.04	105.20	128.17	143.57	156.93	206.85	SVSP, PLA₂, CTL
8	µg TOTAL	4.03	12.10	24.19	36.29	48.38	96.77	145.15	201.50	SVSP. CTL. LAO
8	µg RET	4.03	12.10	24.19	36.29	48.38	83.84	105.48	119.45	SVSP. CTL. LAO
9	µg TOTAL	19.20	57.61	115.22	172.84	230.45	460.90	691.34	960.01	PI-SVMP
9	µg RET	17.92	56.07	113.23	167.29	213.21	278.24	338.59	400.52	PI-SVMP
10	µg TOTAL	44.83	134.48	268.96	403.43	537.91	1075.82	1613.74	2191.51	PIII-SVMP
10	µg RET	43.51	132.72	260.99	351.64	376.24	529.24	629.53	726.54	PIII-SVMP