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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Immunol. 2020 Jan 13;204(5):1201–1213. doi: 10.4049/jimmunol.1901205

Figure 1: Calcitriol regulates Th9 cell development via vitamin D receptor.

Figure 1:

a) Naïve CD4+ T cells were isolated from C57BL/6 mice and activated under different T helper cell differentiating conditions (Th1, Th2, Th9, Th17 and Treg). VDR expression was determined by qPCR and immunoblot normalized against β-actin expression. b) Naive CD4+ T cells were activated in Th9 differentiating conditions with calcitriol (100 nM) and the expression by VDR by Th9 cells was investigated by qPCR and immunoblot. c) Binding of VDR at vitamin D response element upon treatment with calcitriol was tested using ChIP. Graphs depict mean± S.D of 3 independent experiments. *p<0.05 and **p<0.01 when compared to Teff (a) and Th9 (b and c).