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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Immunol. 2020 Jan 13;204(5):1201–1213. doi: 10.4049/jimmunol.1901205

Figure 2: Calcitriol downregulates Th9 cell development.

Figure 2:

(a) Th9 cells were treated with different doses of calcitriol (10, 50 and 100 nM) and the frequency of IL-9+ cells was determined by flow cytometry. FACS plots depict the representative figure for the effect of different doses of calcitriol on the number of IL-9+ cells in Th9-differentiating condition. The graph represents number of IL-9 secreting Th9 cells when treated with different doses of calcitriol from at least three independent experiments. b) and c) Th9 cells were treated with calcitriol (100 nM) and the expression of Il9 as well as the amount of IL-9 secreted by Th9 cells was assessed by qPCR and ELISA. d) Murine vitamin D receptor was ectopically expressed in Th9 cells by retroviral transduction and the number of IL-9+ cells was detected by flow cytometry. Graphs represent mean± S.D of 3 independent experiments. *p<0.05 and **p<0.01 when compared to Th9 cells.