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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Immunol. 2020 Jan 13;204(5):1201–1213. doi: 10.4049/jimmunol.1901205

Figure 4: Vitamin D receptor associates with PU.1 and impairs the binding of PU.1 at Il9 gene.

Figure 4:

a) The three conserved non-coding sequences on Il9 gene have been depicted. The binding of PU.1 at the three CNS has been described. b) Top panel: HEK293T cells were transfected with VDR and PU.1-expressing plasmids, followed by immunoprecipitation with PU.1, probing against VDR. Bottom panel: Immunoprecipitation with VDR and blotting against PU.1 upon transfection of HEK293T cells with VDR and PU.1-expressing plasmids. c) Top panel: Th9 cells treated with calcitriol were immunoprecipitated with VDR and probed against PU.1. Bottom panel: Immunoprecipitation of nuclear lysate from calcitriol treated Th9 cells with PU.1, followed blotting against VDR.