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. 2020 Apr 7;9:e51712. doi: 10.7554/eLife.51712

Figure 4. Identification of known aliphatic glucosinolate biosynthetic genes and glucosinolate-modifying genes from Arabidopsis in Erysimum cheiranthoides.

Aliphatic glucosinolates are synthesized from methionine by a series of enzymes, while additional enzymes are responsible for aliphatic glucosinolate modifications (black box). Red square brackets indicate where gene copy numbers differ between Arabidopsis and E. cheiranthoides, or where gene copies could not be matched unambiguously between species. Glucosinolates with names highlighted in blue were identified in Erysimum cheiranthoides var. Elbtalaue. Abbreviations: branched-chain aminotransferase (BCAT); bile acid transporter (BAT); methylthioalkylmalate synthase (MAM); isopropylmalate isomerase (IPMI); large subunit (LSU); small subunit (SSU); isopropylmalate dehydrogenase(IPMDH); cytochrome P450 monooxygenase (CYP); glutathione S-transferase F (GSTF); glutathione S-transferase Tau (GSTU); glutathione (GSH); γ-glutamyl peptidase 1 (GGP1); SUPERROOT 1 C-S lyase (SUR1); UDP-dependent glycosyltransferase (UGT); sulfotransferase (SOT); flavin monooxygenase (FMO); glucosinolate oxoglutarate-dependent dioxygenase (AOP); 3-butenyl glucosinolate 2-hydroxylase (GS-OH).

Figure 4.

Figure 4—figure supplement 1. Phylogeny of branched-chain aminotransferase (BCAT) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 1.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 2. Phylogeny of bile acid transporter (BAT) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 2.

The tree was generated using the Hasegawa-Kishino-Yano model with evolutionary rates modelled by Gamma distribution with invariant sites with five rate categories.
Figure 4—figure supplement 3. Phylogeny of methylthioalkylmalate synthase (MAM) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 3.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 4. Phylogeny of isopropylmalate isomerase (IPMI) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 4.

The tree was generated using the Kimura 2-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 5. Phylogeny of isopropylmalate dehydrogenase (IPMDH) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 5.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 6. Phylogeny of flavin monooxygenase (FMO GS-OX) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 6.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 7. Phylogeny of glucosinolate oxoglutarate-dependent dioxygenase (AOP) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 7.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 8. Phylogeny of 3-butenyl glucosinolate 2-hydroxylase (GS-OH) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 8.

The tree was generated using the Tamura 3-parameter model.
Figure 4—figure supplement 9. Phylogeny of E. cheiranthoides, A. thaliana, and B. oleracea genes with similarity glucoraphasitin synthase (GRS) from R. sativus.

Figure 4—figure supplement 9.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 10. Phylogeny of myrosinase (thioglucoside glucohydrolase, TGG) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 10.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 11. Phylogeny of epithiospecifier protein (ESP) and nitrile specifier protein (NSP) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 11.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.
Figure 4—figure supplement 12. Phylogeny of epithiospecifier modifier (ESM) genes from E. cheiranthoides, A. thaliana, and B. oleracea.

Figure 4—figure supplement 12.

The tree was generated using the Tamura 3-parameter model with evolutionary rates modelled by Gamma distribution with five rate categories.