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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2019 Nov 13;33(3):446–457. doi: 10.1111/pcmr.12837

Figure 4. MX2 downregulation perturbs cell cycle and reduces proliferation in a subset of melanoma lines.

Figure 4.

a) Trypan Blue dye exclusion viability test 72 hours post transfection with two independent siRNAs for MX2 (#1 siMX2 and #2 siMX2) and negative control (siCTR). Viability counts are normalized to negative control. Results are expressed as mean ±SE of three independent experiments. Two-tailed paired t-test was used to test statistical significance. * p<0.05. b) Immunoblot analysis of affected apoptosis and proliferation associated proteins upon MX2 knockdown. GAPDH was used as a loading control c) Evaluation of cell cycle distribution by flow cytometry using propidium iodide staining. Cells were transfected with #1 siMX2, #2 siMX2 and siCTR 48 hours prior to flow cytometry analysis. Bar graphs represent percentages of cells in different cell cycle phases (average from three independent experiments ±SE). d) Immunoblot analysis of proteins involved in G1/S transition of the cell cycle. e) Oscillation of MX2 protein level during cell cycle was examined by releasing MM382 cells synchronized in G1/S phase from double thymidine block.