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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Mar 12;40(5):1256–1274. doi: 10.1161/ATVBAHA.120.314147

Figure 1. Species-specific role of LMCD1 in thrombin-induced VSMCs proliferation and migration.

Figure 1.

A-D. Quiescent HASMCs (A & B) or MASMCs (C & D) were treated with and without thrombin (0.5 U/ml) for the indicated time periods, and either total RNA was isolated and analyzed by RT-PCR for LMCD1 and β-actin mRNA levels (A & C) using their specific primers or cell extracts were prepared and analyzed by Western blotting for LMCD1 and β-actin protein levels (B & D) using their specific antibodies. HASMCs (E & G) or MASMCs (F & H) that were transfected with siControl or siLMCD1 (100 nM) were quiesced and tested for thrombin (0.5 U/ml)-induced DNA synthesis (E & F) and cell migration (G & H). The bar graphs represent Mean ± S.D. values of three independent experiments. *, p < 0.05 versus siControl + vehicle; **, p < 0.05 versus siControl + Thrombin.