Fig. 1.

Protoplast isolation from buckwheat. A. Soil-grown buckwheat seedlings cultivated for 7 days after germination. B. A representative seedling used for protoplast isolation. C. Cotyledons placed on 3M adhering tape. D. A Cotyledon in which the adaxial epidermis was removed by peeling using the tape-sandwich method. E. The cotyledons attached to tape are incubated in a 50-ml tube to digest cell walls. F. Longitudinally cut hypocotyl. G. Segments of longitudinally cut hypocotyls for protoplast isolation. H. Hypocotyl segments digested with cellulose solution in a 50-ml tube. Scale bars = 5 cm (A and B) and 1 cm (C to H).