FIGURE 6.

Effect of 1,000 mg/L ficin (marked as F1000) and 32 mg/L dalbavancin (marked as D32) on preformed MRSA4 biofilms as measured by CV staining and viable CFU counting. (A) MRSA4 biofilm quantification by CV in flat-bottom 96-well Ibidi plates, performed by duplicate. (B) Biofilm formation capacity after different treatments and CV staining (measured as optical density). Data show average values from three biological replicates. (C) Bacterial viability of biofilm and planktonic MRSA4 cells after treatment by ficin, dalbavancin, and their combination for 24 h. Data show the average of log CFUs from three replicates. Statistical significance was assessed by t-test; asterisks indicate *p ≤ 0.05 and ***p ≤ 0.001.