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. 2020 Apr 17;11:708. doi: 10.3389/fmicb.2020.00708

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Copyright © 2020 Ren, Chen, Jiang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ExPEC XM O2:K1:H7 infection leads to COX-2 up-regulation in macrophages. (A) The mRNA expression level of COX-2 in RAW264.7 and BMDM macrophages under ExPEC XM O2:K1:H7 infection were analyzed by qRT-PCR. qRT-PCR results were normalized to the housekeeping gene GAPDH. (B) The protein expression level of COX-2 in RAW264.7 and BMDM macrophages after ExPEC XM O2:K1:H7 infection at MOI 10 was analyzed by western-blot. Western-blot results were also analyzed with GAPDH as a loading control. The qRT-PCR represented the mean ± SD of three independent experiments.