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. 2020 Apr 17;11:708. doi: 10.3389/fmicb.2020.00708

FIGURE 3.

FIGURE 3

COX-2 up-regulation in ExPEC XM O2:K1:H7-infected macrophages through TLR4 mediated p38 and ERK1/2 MAPK pathway. (A) The TLR4 mRNA level of macrophages with or without ExPEC XM O2:K1:H7 treatment at 1 h was assayed by qRT-PCR. (B) Western blot analysis of COX-2 expression levels from cell lysates of RAW264.7 macrophages treated with or without TLR4 inhibitor (TAK-242) accompanied by ExPEC XM O2:K1:H7 infection for 6 h. (C) Western blot analysis of COX-2 expression levels from cell lysates of RAW264.7 macrophages treated with or without p38 MAPK inhibitor [SB202190 (FHPI)] and ERK1/2 MAPK inhibitor (SCH772984), accompanied by ExPEC XM O2:K1:H7 infection for 6 h. (D) Western blot analysis of indicated proteins with specific antibodies from cell lysates of RAW264.7 macrophages treated with or without 10 μM TAK-242, accompanied by ExPEC infection for 1 h.