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. 2020 Apr 17;11:708. doi: 10.3389/fmicb.2020.00708

FIGURE 4.

FIGURE 4

COX-2 inhibition attenuated autophagy in ExPEC XM O2:K1:H7-infected macrophages in vitro and autophagy formation contribute ExPEC XM O2:K1:H7 clearance. (A) Western-blot analysis of LC3 expression of RAW264.7 macrophages treated with COX-2 inhibitor NS398 or DMSO at 6 h after infection with ExPEC XM O2:K1:H7 at MOI 10. (B) RAW264.7 macrophages cells pretreated with autophagy inhibitor 3-methyladenine (3-MA; 10 μM), autophagy inducer rapamycin (rap; 10 μM) and DMSO or PBS vehicle were infected with ExPEC XM O2:K1:H7 at MOI 10, the numbers of intracellular viable bacteria were collected at the indicated times and determined by plate counting assay. Statistical significance was determined by using unpaired t test (*P < 0.05).