. 2020 Apr 17;8:242. doi: 10.3389/fbioe.2020.00242

TABLE 2.

Quantitative variability in polar metabolites of A. thaliana leaves during plant pathogen nanoparticles interaction.

Rt (Min)	Metabolites	Fragmentation patterns	Control (μg/g)	SNP (μg/g)	AB (μg/g)	SNP + AB (μg/g)
6.79	Ethylene glycol	m/z 206,193,191,147,103,73	0.710 ± 0.074^b	0.270 ± 0.028^d	0.500 ± 0.052^c	1.370 ± 0.143^a
16.07	Glycerol	m/z 308,293,219,205,148,117,75,73,55	1.360 ± 0.142^d	2.590 ± 0.270^c	5.990 ± 0.623^b	9.470 ± 0.986 ^a
16.72	Maleic acid	m/z 260,247,245,147,75,73,66	ND^b	0.290 ± 0.030^a	ND^b	ND^b
16.95	Succinic acid	m/z 262,249,247,172,148,129,75,73,55	1.460 ± 0.152^a	1.300 ± 0.135^a	ND^b	ND^b
17.63	Glyceric acid	m/z 332,308,292,205,189,147,103,73	0.900 ± 0.094^b	2.140 ± 0.223^a	ND^c	ND^c
17.89	Fumaric acid	m/z 260,248,217,147,133,75,73	ND^b	11.350 ± 1.18^a	ND^b	ND^b
21.77	Malic acid	m/z 350,335,307,245,148,133,73,55	ND^b	7.680 ± 0.799^a	ND^b	ND^b
22.39	Erythritol	m/z 410,348,307,217,205,189,147,117,103,73	0.570 ± 0.059^a	0.251 ± 0.026^c	ND^c	0.240 ± 0.02^b
23.26	Erythronic acid	m/z 424,409,379,294,220,149,117,73	0.280 ± 0.029^a	0.320 ± 0.03^a	ND^b	ND^b
23.68	L-Threonic acid	m/z 424,409,379,294,220,189,147,117,73	1.060 ± 0.110^b	3.120 ± 0.325^a	ND^c	ND^c
26.12	D-Xylose	m/z 467,421,362,307,147,103,73,59	1.520 ± 0.158^a	0.940 ± 0.098^c	1.220 ± 0.127^b	0.970 ± 0.10^c
26.48	D-ribose	m/z 467,467,362,218,189,103,73	4.350 ± 0.453^a	0.410 ± 0.043^bc	0.660 ± 0.069^b	ND^c
27.38	Xylitol	m/z 512,395,307,217,147,103,73	1.030 ± 0.107 ^b	0.300 ± 0.031^c	3.050 ± 0.317^a	1.240 ± 0.129^b
28.36	Ribonic acid	m/z 526,511,421,333,292,217,147,103,73	1.680 ± 0.175^b	2.220 ± 0.231^a	ND^c	ND^c
28.56	Galactonic acid	m/z 628,511,393,292,217,189,147,103,73	ND^b	1.860 ± 0.194^a	ND^b	ND^b
30.65	D-Fructose (MeOX1)	m/z 569,408,307,263,217,173,147,103,73	2.100 ± 0.219^c	8.160 ± 0.849^b	16.840 ± 1.753^a	ND^d
30.86	D-Fructose (MeOX2)	m/z 569,408,307,263,217,173,147,103,73	ND^c	5.840 ± 0.608^b	11.220 ± 1.168^a	ND^c
31.13	D-Glucose (MeOX2)	m/z 569,466,390,319,217,160,147,73	2.480 ± 0.258^c	14.130 ± 1.47^b	17.170 ± 1.787^a	1.160 ± 0.121^c
31.49	D-Galactose	m/z 569,376,217,160,147,103,73	ND^b	4.200 ± 0.437 ^a	4.680 ± 0.487 ^a	ND^b
31.76	D-Glucitol	m/z 614,422,346,320,217,157,103,73	ND^c	0.430 ± 0.045 ^c	12.370 ± 1.288^a	4.290 ± 0.447^b
32.03	Ribitol	m/z 512,379,218,157,103,73,59	ND^b	ND^b	1.810 ± 0.188^a	ND^b
32.18	Gulonic acid	m/z 466,361,218,147,129,75,73	1.710 ± 0.178^b	0.150 ± 0.016^c	2.400 ± 0.250^a	1.080 ± 0.112^b
33.16	Gluconic acid (Di TMS) (6TMS)	m/z 628,437,334,292,277,147,73	ND^b	5.220 ± 0.543 ^a	0.420 ± 0.044^b	ND^b
34.71	Inositol	m/z 612,508,362,337,217,191,147,73	11.550 ± 1.202^b	3.160 ± 0.329^c	1.460 ± 0.360^d	29.910 ± 3.113^a
38.75	α-D Galactopyranoside (6TMS)	m/z 686,362,217,204,191,147,73	2.320 ± 0.241^b	2.480 ± 0.258^b	3.200 ± 0.333 ^b	18.350 ± 1.910^a

Mean values ± SD of μg/g of the dry weight of samples. ND, not detected; a, b, c, and d denote statistical significance P < 0.05. Cont, control; SNP, biogenic silver nanoparticles alone; AB, A. brassicicola-infected plants; AB + SNP, A. brassicicola-infected, treated with SNP.