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. 2020 Apr 17;8:327. doi: 10.3389/fbioe.2020.00327

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Copyright © 2020 Nossin, Farrell, Koevoet, Somoza, Caplan, Brachvogel and van Osch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chondrogenic differentiation of bone marrow derived mesenchymal stem cells (BMSC). (A) Histological and Immunohistological staining of day 21 chondrogenically differentiated BMSC-pellets of the donors for Collagen type II, Thionine and Collagen type X. (B) qPCR Gene-expression analysis of chondrocyte and hypertrophy marker genes in day 21 chondrogenically differentiated BMSCs. Raw average Ct value for COL2A1 = 20.54; COL10A1 = 20.99; ALPL = 28.19.