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. 2020 Apr 17;10:545. doi: 10.3389/fonc.2020.00545

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Copyright © 2020 Wencong, Jinghan, Yong, Jianyang, Bin, Qingbao, Chen and Xiaoqing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FOXK1 expression was upregulated in GBCs and correlated with overall survival. (A,B) Relative mRNA levels of FOXK1 in 42 paired human GBC tissues and adjacent normal tissues were determined by real-time qPCR. Gene expression results were normalized by GAPDH. (C) FOXK1 protein expression in 10 paired GBC tissues and their corresponding non-tumor tissues was determined by Western blot assay. (D) Representative images of IHC staining of FOXK1 in GBC tissues and non-tumor (cholecystitis) tissues (scale bar = 20 μm). (E) Percentage of cases with different staining intensity of FOXK1 in in the cholecystitis or GBC tissues. (F) Kaplan-Meier overall survival curve of GBC patients based on FOXK1 expression.