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. 2020 Apr 17;10:545. doi: 10.3389/fonc.2020.00545

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Copyright © 2020 Wencong, Jinghan, Yong, Jianyang, Bin, Qingbao, Chen and Xiaoqing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FOXK1 promotes GBC cell proliferation in vitro. (A) The protein levels of FOXK1 in four GBC cell lines. (B) Western blot analysis of FOXK1 expression in FOXK1-depleting GBC-SD and NOZ cells and FOXK1-overexpressingSGC-996 cells. GAPDH was used as the loading control. (C) The cell proliferation was determined by CCK-8 assays at various time points. (D) Effects of FOXK1 on the proliferation of GBC-SD, NOZ, and SGC-996 cells were assessed by the colony-forming assay. (E) The numbers of colonies were counted and depicted in the bar chart. Data shows the mean ± SD for three independent experiments. (*P < 0.05, **P < 0.01, and ***P < 0.001).