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. 2020 Apr 17;10:545. doi: 10.3389/fonc.2020.00545

Figure 5.

Figure 5

FOXK1 promotes tumor invasion and metastasis by inducing EMT. (A,B) For wound-healing assay, cell mobility was inhibited in FOXK1-depleting GBC-SD and NOZ cells. Overexpression of FOXK1 in SGC-996 cells had the opposite effects. Relative migration rate was shown in the bar chart. (C,D) Effects of FOXK1 on the migration and invasion of FOXK1-depleting GBC-SD and NOZ cells and FOXK1-overexpressingSGC-996 cells were determined by the transwell migration assay and matrigel invasion assay, respectively. The number of invaded cells were calculated and depicted in the bar chart. (E) The protein expression of N-cadherin, vimentin and E-cadherin in GBC cells was examined by Western blotting. (F) Representative images of H&E staining of lung metastases in each group. The number of metastatic nodules in the lung was calculated and compared in the bar chart. (*P < 0.05, **P < 0.01, and ***P < 0.001).